What is Small RNA & miRNA Sequencing

The study of RNA transcription and interference has grown tremendously since the discovery of microRNA. The term “small RNA” has now come to include many types of RNA, such as microRNA (miRNA), short interfering RNA (siRNA), piwi-interacting RNA (piRNA), and more.

Ion Torrent™ next-generation sequencing systems provide sequencing-based analysis of all known and novel small RNA transcripts in a strand-specific, hypothesis-free fashion. Both the Ion Proton™ and Ion PGM™ Systems offer a range of integrated, optimized reagents and workflows for sample preparation through data analysis in your research.

Features include:

Flexible sample input—analyze small RNA from sample types such as FFPE tissue, starting with as little as 5 ng Increased dynamic range

Increased dynamic range—quantify transcripts more accurately with increased sequencing depth and a much higher dynamic range than with a microarray

Precise identification—differentiate between closely related transcript species

Preserved strand orientation—map small RNA exactly to the strand it is derived from

Novel RNAs—detect more unmapped small RNAs and isoforms