How Genome Sequence Scanning perform well?

The first capability involves bacterial population profiling for microbiome analyses. Recent studies show that humans harbor vast and diverse populations of microorganisms that are essential to life and health. Research is now focused on correlating health and disease to the composition and changes in an individual’s microbiome. With more than 1,000 publications in 2011, microbiome analysis is one of the fastest growing fields of investigation.

Current techniques for profiling the microbiome in a specimen are labor intensive and can take several weeks. Because Genome Sequence Scanning™ can rapidly detect and quantitate bacterial strains from complex samples, the technology has the potential to become a mainstay of microbiome research.

The second capability involves rapid, high-resolution strain typing. Molecular typing of bacteria is widely used to understand how differences in genomic profile correlate to phenotypic characteristics such as pathogenicity.

Current typing techniques such as pulsed field gel electrophoresis (PFGE) take several days and results are difficult to compare among different operators and laboratories. GSS enables high resolution typing to be performed in hours and with a consistent data format that allows genomic structures to be readily compared.

Can Proteins Interact with DNA and RNA to Influence Nucleic Acid?

Due to the fact that nucleic acids carry genetic information and that proteins regulate various life processes, they are considered to be two of the most important biomolecules in any living organism. In addition, their interactions play a crucial role in most biological processes, which include everything from replication,transcription and recombination to enzymatic eventsusing nucleic acids as substrates. Taking all of these things into consideration, it is not surprising why protein-nucleic acids interactions have been the subject of intensive research for the past few years.

Submit a question and The Protein Man and his team at G-Biosciences will provide you an answer or a resource to aid you.  Alternatively other scientists or science enthusiasts can also provide answers, suggestions and comments.

Student need to know How Genomic DNA extraction works?

While there may be a number of ways by which you can successfully isolate your genomic DNA from your sample, your final choice of which genomic DNA extraction protocol to use will ultimately depend on several factors which includes the following:

• the molecular weight of the DNA of interest;
• quantity and purity required to facilitate downstream applications;
• ease or complexity of your chosen method; 
• time requirements; and 
• budgetary constraints.

Genomic DNA can be separated from all other cellular components by simply following these three basic steps:

Disruption and cell lysis. In extracting your genomic DNA from the sample, you need to break down the cell walls that protect the DNA by using enzymes such as lysozyme and proteinase K or by using physical methods such as manual grinding (mortar and pestle method), freeze-thaw technique, sonication, liquid homogenization and/or mechanical disruption with the use of a Waring blender or a polytron. You can also use bead beating (using 0.1 mm glass beads or 0.15 mm garnet beads) to release your genomic DNA from your cell lysate.

Undertanding Present DNA Stretching Technology at APS Physics Meeting

PathoGenetix, Inc., a commercial-stage developer of an automated system for rapid bacterial identification, has been invited to present new research at the 2014 American Physical Society (APS) March Meeting next week in Denver, Colorado, demonstrating the integration of multiple mechanisms in its proprietary microfluidic funnels which enables optimal stretching and scanning of large DNA molecules for rapid analysis and identification of bacteria. Entitled “High-throughput DNA Stretching in Continuous Elongational Flow for Genome Sequence Scanning,” the presentation adds to the growing body of research demonstrating and detailing the company’s Genome Sequence Scanning™ (GSS™) technology, which enables rapid and reliable identification and strain typing of bacterial pathogens. The APS Physics March Meeting is expected to draw close to 10,000 physicists, scientists and students this year, with more than 600 sessions describing research from industry, universities and major laboratories from around the world.

PathoGenetix’s Genome Sequence Scanning (GSS) is a bacterial identification technology that detects sequence-specific fluorescent tags on long DNA molecules that have been extracted and purified directly from biological samples. Key to the efficacy and sensitivity of the GSS technology is the microfluidic funnel used for high-throughput stretching and scanning of long strands of single DNA molecules. In the proprietary GSS detection funnels, purified and tagged DNA molecules flow in a linear conformation at high speed past a series of lasers and optical sensors, which record the length and pattern of the labels on each DNA fragment. The labels create a barcode of the DNA in the sample, which is compared to an onboard database of barcodes to identify the serotype/strain type for the organism.

What is Exome Sequencing Generation Sequencing

Exome sequencing is a targeted sequencing approach that is restricted to the protein-coding regions of genomes. The exome is estimated to encompass approximately 1% of the genome, yet contains approximately 85% of disease-causing mutations [1]. For genetic researchers trying to identify the genes implicated in over 6,800 rare diseases [2], exome sequencing enables rapid, cost-effective identification of common single nucleotide variants (SNVs), copy number variations (CNVs), and small insertions or deletions (indels), as well as rare de novo mutations that may explain the heritability of Mendelian and complex disorders [3].

 Rapid, accurate exome sequencing with the Ion AmpliSeq™ Exome workflow
Leveraging the ultrahigh-multiplex PCR approach of Ion AmpliSeq™ technology and the superior accuracy of the new Ion Proton™ Hi-Q™ Sequencing Solution, the Ion AmpliSeq™ Exome RDY Kit allows for rapid, accurate sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.

Exome sequencing services: Ion Torrent™ Certified Service Providers
The Ion Torrent™ Certified Service Provider program is a global network of validated service providers that enable researchers in small- and medium-sized laboratories to get high-quality exome and transcriptome sequencing data generated on the Ion Proton™ System from small amounts of input material with very fast turnaround.

You know reason Car Insurance So Expensive For Teens?

At sixteen years old the universes of dating, working low maintenance and above all driving open up to youth. The gas and hit the legs, spastic kid is a young lady, and the way to hand, and we anticipate opportunity!

In any case, it isn't generally free by any means. Shockingly, regardless of the possibility that a teen is dependable and driving a safe vehicle, the premiums on their arrangement will be high. Most children aren't going to take no for an answer when you decline to give them a chance to drive on the grounds that it is so extravagant to protect them.

Teens cost more to safeguard. They put a larger number of requests on their protection than whatever other demographic gathering That permits the organization to earn back the original investment on pay-out and working expenses, with somewhat over as benefit for the organization and its investors and the way protection works is by spreading hazard over gathering of comparative people, charging a cost.

The insurance agency is going to decide how likely the individual being protected is going to need a payout and the amount it would be, and afterward they utilize that add up to decide how much the premium would be. Under the heading of "measurements" and this range of math and science falls.

This is the spot where the accuracy of the numbers crushes protection purchasers. More than 10 different drivers have mischance insights of drivers in their teens. It's not simply somewhat more, its a great deal more. A 16-year-old driver is three times as liable to bite the dust in a car accident than whatever other driver, and that demise rate enhances gradually through the following couple of years.

Teens oblige pay-out than different drivers in light of the fact that the have more wrecks Most wrecks that include 15 – 17 year old new drivers can cost up to 34 billion dollars. That is right, billion. This is a huge astonishment asked for enough cash to pay for additional protection is not awful 34 billion dollars.

Next your teen argues: "I'm an incredible driver however! I went to driver's ed and even extra courses, and I'm generally mindful… "

Custom curriculum for crises, the measurements enhance and the uplifting news is that, initially, on the off chance that you tyke has taken driver's ed lessons. They improve if your youthful defers driving for around a year's chance. They are likely more secure, thus organizations consider such things when the expense of protection is computed. This is a youthful driver training and the fitting bit of additional time and extraordinary preparing to locate a dependable organization to concede lower interest rates is not unimaginable.

To locate the best scope for your kid you have to do the vital exploration. Survey arrangements, meet with operators, and dependably make certain to get offers. A standout amongst the most effective approaches to get cites for teen protection is to inquiry online from a few separate organizations.

First Reason Most People Don’t Buy Life Insurance to protect in US & Cananda

It's excessively costly! is the regular abstain when Americans are inquired as to why they don't get the extra security insurance they require. Be that as it may and its an enormous however 80% overestimate the amount it costs.

For the fifth year, Life Happens banded together with LIMRA to create the Insurance Barometer Study, which takes a gander at shopper patterns and customers' observations in regards to disaster protection, retirement and their budgetary prosperity.

"We've reliably seen throughout the most recent five years that buyers think disaster protection is more extravagant than it truly is," says Marvin Feldman, CLU, ChFC, RFC, president and CEO of Life Happens. "We have to help teach people in general about how reasonable life coverage can be."

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