How does Methylated DNA Enrichment work?

MethylMine Methylated DNA Enrichment Kit enables superior enrichment and differential fractionation of double-stranded DNA based on CpG methylation density, with increased sensitivity over antibody-based methods. Fractionation permits important comparisons between samples and enables researchers to focus analysis on only the methylation densities of interest.

 Advantages of MethylMiner™ Methylated DNA Enrichment Kit include:

Partitioning with high affinity binding—at least 4-fold greater sensitivity than antibody-based methods

Fractionation based on CpG methylation density— ds DNA capture is achieved with MBD2 protein and facilitates ligation of double-stranded adaptors for next-generation sequencing

Rapid and easy elution with salt eliminates the need for proteinase K treatment and phenol:chloroform extraction

Precise answers—fractionated DNA permits distinctions to be made regarding methylation status and density

Fast protocol—completed in less than 4 hours 

Easy—simple handling with Dynabeads® - the gold standard in magnetic beads