What is BISUPHITE CONVERSION OF DNA?

DNA methylation is a phenomenon involved in many diseases, the most frequently studied of which is cancer. In mammals, methylation occurs at cytosine bases which are followed by a guanine base (CpG), principally in the form of 5-methylcytosine

Bisulphite conversion produces two distinct, non-complementary DNA strands.
Bisulphite conversion produces two distinct, non-complementary DNA strands.
Following PCR amplification of one of these strands, the unmethylated cytosines are “replaced” by thyamines. The percentage of cytosines remaining represents the percentage of methylation at a given position.

The French Centre National de Génotypage (CNG), which is part of the Institute of Genomics at the CEA in Evry (near Paris), and the Toulouse platform, both have the necessary know-how for optimal performance of this technique.