MeDIP is a technique that uses anti-methylcytosine antibodies which are coupled to magnetic beads to immunoprecipitate the regions that are sufficiently methylated.
JPEG - 20.3 kbSample DNA is fragmented by sonication then antibodies are added. Once these have bonded to the methylcytosines, the methylated fragments are collected using a magnet, thus removing from the sample any unmethylated or weakly methylated fragments: the sample has thus been enriched with methylated sequences and is ready to be analysed using a sequencing technique. For better reproducibility, it is recommended to use a robot to perform MeDIP.
The French National Genotyping Centre (CNG), which is part of the Institute of Genomics at the CEA in Evry (near Paris), and the Toulouse platform, both have the necessary equipment to optimise this pre-sequencing step.
