NaOH extraction. Known as the "quick-and-dirty" method of preparing DNA, this technique is quite easy to implement and is usually sufficient for most applications. All you need to do is to incubate your cell lysate at high temperatures or subject it to proteinase K digestion and you can have your genomic DNA ready for downstream applications. However, since DNA extracted using this method may contain high levels of contamination, it should not be stored for future use.
Phenol-chloroform extraction. This technique uses organic solvents to extract contaminants from your lysate while the DNA is recovered from the aqueous phase through alcohol precipitation. This may be the most conventional technique of extracting highly purified genomic DNA from a sample but it can also be quite time consuming and may not give reproducible yields.
Silica-based methods. Genomic DNA can easily be extracted from mammalian cells and tissues as well as from mouse tail, E. coli cells and yeast by using silica-based methods. By choosing to use this genomic DNA extraction protocol, you can get your ready-to-use DNA in less than 15 minutes using a spin column based centrifugation procedure. The extracted DNA using this method has an average size of 20 to 30 kb and is ideal for use in PCR, southern blotting analysis and restriction enzyme digestion.
